三氧化二砷抑制DNA损伤修复的放射增敏

doi:10.13491/j.issn.1004-714X.2024.04.013

中国辐射卫生

2024, Vol. 33

Issue (4): 426-434 DOI: 10.13491/j.issn.1004-714X.2024.04.013

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三氧化二砷抑制DNA损伤修复的放射增敏

高兴兴^1,2, 惠慧^1,2, 任洪荣^1,2, 周云^1,2

1. 徐州医科大学附属第一临床医学院江苏徐州 221000;
2. 徐州市中心医院江苏徐州 221000

Arsenic trioxide achieves radiosensitization by inhibiting DNA damage repair

GAO Xingxing^1,2, HUI Hui^1,2, REN Hongrong^1,2, ZHOU Yun^1,2

1. Department of Affiliated The First Clinical Medical College of Xuzhou Medical University, Xuzhou 221000 China;
2. Xuzhou Central Hospital, Xuzhou 221000 China

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摘要目的探讨三氧化二砷（ATO）在宫颈癌中的放射增敏作用，并进一步探索其相关的DNA损伤修复机制。方法体外培养人宫颈癌细胞（Siha、Hela细胞），给予不同浓度的ATO处理，利用CCK-8实验检测细胞增殖情况；将细胞分为4个组：对照（Control）组、放疗（IR）组、ATO组、放疗 + 给药（IR + ATO）组，平板克隆实验计算放射增敏比；流式细胞术检测细胞周期及凋亡情况；免疫荧光检测γH2AX的表达；蛋白免疫印记法（Western blot）检测Cyclin B1、PTEN、RAD51的表达水平；逆转录聚合酶链式反应（qPCR）检测RAD51的mRNA水平。结果 CCK-8结果显示1 μM及更高浓度的ATO可以显著抑制Siha及Hela细胞增殖。平板克隆结果显示，ATO对宫颈癌具有放射增敏作用，放射增敏比（SER）分别为1.37、1.30。流式细胞术结果显示放疗 + 给药组的细胞周期阻滞比例较对照组显著增高（P < 0.001)；放疗 + 给药组的凋亡率较对照组明显升高（P < 0.01)。蛋白免疫印迹法结果显示放疗 + 给药组PTEN、RAD51蛋白的表达水平降低（P < 0.05)、Cyclin B1蛋白的表达水平升高（P < 0.05)，差异具有统计学意义。结论 ATO通过消耗PTEN来阻滞DNA同源重组修复途径，从而在宫颈癌中达到放射增敏的效果。

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	高兴兴
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关键词 ：三氧化二砷, 宫颈癌, DNA损伤修复, 放射增敏

收稿日期: 2024-01-26

PACS:

X591

基金资助:江苏省妇幼健康项目（F202035），难治型妇科恶性肿瘤 SBRT治疗

通讯作者: 周云，E-mail：zhouyunxzch@yeah.net E-mail: zhouyunxzch@yeah.net

作者简介: 高兴兴（1998—），女，山东泰安人，硕士研究生，从事主要研究方向为妇科肿瘤的放射治疗工作。E-mail：gao1922103@126.com

引用本文:

高兴兴, 惠慧, 任洪荣, 周云. 三氧化二砷抑制DNA损伤修复的放射增敏[J]. 中国辐射卫生, 2024, 33(4): 426-434.
GAO Xingxing, HUI Hui, REN Hongrong, ZHOU Yun. Arsenic trioxide achieves radiosensitization by inhibiting DNA damage repair. Chinese Journal of Radiological Health, 2024, 33(4): 426-434.

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http://www.zgfsws.com/CN/10.13491/j.issn.1004-714X.2024.04.013 或 http://www.zgfsws.com/CN/Y2024/V33/I4/426

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