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| Study of the Dose-effect Relationship of γ-H2AX Radiation |
| CUI Shuang-shuang, FAN Ya-guang, SUN Zhi-juan, et al |
| Institnte of Radiation Medicina, Chinese of Medical Sciences and Peking Union Medical College, Tianjing 300192 China |
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Abstract Objective By using laser scanning confocal microscopy (LSCM)to test the dose-effects relationship between ionizing radiation intensity and quantity of the γ-H2AX in vivo and in vitro respectively,and the consistency relationship between the vivo and vitro retrial.Methods To irradiate the peripheral blood from Wister female rats by 137Cs at 7 with different doses (0、0.5、1、2、2.54、4、6、8Gy)extract the lymphocytes from the peripheral blood and detect the dose-effects relationship between irradiation intensity and number of γ-H2AX foci.Results There are good dose-effects relationships between the irradiation and foci number both in vivo and in vitro,which are linear,Yvivo=0.096+0.13X;Yvitro=0.040+0.21X.And there is good consistency (R=0.98)between the γ-H2AX in vivo and in vitro.Conclusions γ-H2AX has the possibility for clinical trial as an indicator,and we can use vitro trials in place of the vivo trails to evaluate the dose people received.
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Received: 21 June 2009
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