|
|
|
| Analysis of micronucleus rate in 394 radiation workers with cytokinesis-block micronucleus method |
| LIU Haixiang1, ZHAO Hua2, GAO Yu3, ZHANG Jie1, ZHANG Xueqing1, XIA Ying1 |
1. Medical Examination Center, Beijing Nuclear Industry Hospital, Beijing 100045 China;
2. China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088 China;
3. Henan Key Laboratory of Medicine on Radiobiology and Epidemiology, Third People’s Hospital of Henan Province, Henan Province Occupational Disease Hospital, Zhengzhou 450052 China |
|
|
|
|
Abstract Objective To analyze the micronucleus rate of radiation workers and to provide accurate occupational health monitoring basis in radiation workers exposed to low-level ionizing radiation for a long time. Methods The radiation group consisted of 353 radiation workers who had been exposed to ionizing radiation during work, while the control group consisted of 41 radiation workers who had not yet been exposed to ionizing radiation before work. The cytokinesis-block micronucleus method was used to determine the micronucleus rate. Results The average micronucleus rate in the radiation group was significantly higher than that in the control group (t = -2.95, P < 0.05). In the radiation group, the micronucleus rate gradually increased with age, and the difference was statistically significant (F = 8.36, P < 0.05). The micronucleus rates of workers with > 10 and > 30 years of service were significantly higher than those of workers with < 10 years of service (χ2 = -44.79, -60.47, P < 0.05). The micronucleus rate in females was significantly higher than that in males (t = 3.93, P < 0.05). The micronucleus rates in the diagnostic radiology group and the industrial detection group were significantly higher than that in the control group (t = 3.51, 3.65, P < 0.05). Conclusion The micronucleus rate has increased among the radiation workers exposed to low-level ionizing radiation for a long time. It is necessary to further strengthen occupational health monitoring and radiation protection education for radiation workers, especially the medical workers that constitute the largest population of radiation exposure workers.
|
|
Received: 06 September 2023
|
|
|
|
|
|
[1] United Nations Scientific Committee on the Effects of Atomic Radiation. Sources and effects of ionizing radiation. Report to the general assembly, with scientific annexes[R]. New York: United Nations, 2000. DOI: 10.18356/49c437f9-en.
[2] The 2007 recommendations of the international commission on radiological protection. ICRP publication 103[J]. Ann ICRP, 2007, 37(2/4): 1-332. DOI: 10.1016/j.icrp.2007.10.003
[3] Charles M. UNSCEAR report 2000: sources and effects of ionizing radiation[J]. J Radiol Prot, 2001, 21(1): 83-86. DOI: 10.1088/0952-4746/21/1/609
[4] 吕玉民. 染色体畸变在急、慢性辐射损伤评估中的意义专家解析[J]. 中国辐射卫生,2019,28(4):349-354,360. DOI: 10.13491/j.issn.1004-714X.2019.04.001
Lv YM. Expert interpretation on the significance of chromosomal aberration in the assessment of acute and chronic radiation damage[J]. Chin J Radiol Health, 2019, 28(4): 349-354,360. DOI: 10.13491/j.issn.1004-714X.2019.04.001
[5] 中华人民共和国国家卫生健康委员会. GBZ/T 328—2023 放射工作人员职业健康检查外周血淋巴细胞微核检测方法与受照剂量估算标准[S]. 北京:中国标准出版社, 2023.
National Health Commission of the People’s Republic of China. GBZ/T 328—2023 Standard for the method of micronucleus detection in lymphocytes on occupational health examination for radiation workers and exposure dose estimation[S]. Beijing: Standards Press of China, 2023.
[6] 中华人民共和国卫生部. GBZ 235—2011 放射工作人员职业健康监护技术规范[S]. 北京: 中国标准出版社, 2011.
Minister of Health of the People’s Republic of China. GBZ 235—2011 Specifications for occupational health surveillance for radiation workers[S]. Beijing: Standards Press of China, 2011.
[7] Fenech M. Cytokinesis-Block micronucleus cytome assay evolution into a more comprehensive method to measure chromosomal instability[J]. Genes, 2020, 11(10): 1203. DOI: 10.3390/genes11101203
[8] 叶淑敏. 放射工作人员735人淋巴细胞微核检测结果分析[J]. 海峡预防医学杂志,2023,29(1):84-86
Ye SM. Analysis of lymphocyte micronucleus in 735 radiation workers[J]. Strait J Prev Med, 2023, 29(1): 84-86
[9] 王志敏. 淄博市放射工作人员外周血淋巴细胞微核率分析[J]. 中国工业医学杂志,2022,35(5):463-465. DOI: 10.13631/j.cnki.zggyyx.2022.05.026
Wang ZM. Analysis of micronucleus rate of peripheral blood lymphocytes of radiological workers in Zibo city[J]. Chin J Ind Med, 2022, 35(5): 463-465. DOI: 10.13631/j.cnki.zggyyx.2022.05.026
[10] 黄磊, 赵士义, 王楠, 等. 天津市2021年放射工作人员职业健康检查结果分析[J]. 辐射防护通讯,2022,42(4/5):2-6. DOI: 10.3969/j.issn.1004-6356.2022.04.002
Huang L, Zhao SY, Wang N, et al. Analysis of occupational health examination results of radiation workers in Tianjin in 2021[J]. Radiat Prot Bull, 2022, 42(4/5): 2-6. DOI: 10.3969/j.issn.1004-6356.2022.04.002
[11] 王静, 周学文, 梅丹, 等. 湖北省放射工作人员外周血淋巴细胞微核检测结果分析[J]. 工业卫生与职业病,2023,49(5):442-444. DOI: 10.13692/j.cnki.gywsyzyb.2023.05.015
Wang J, Zhou XW, Mei D, et al. Analysis of micronucleus test results of peripheral blood lymphocytes among radiation workers in Hubei Province[J]. Ind Health Occup Dis, 2023, 49(5): 442-444. DOI: 10.13692/j.cnki.gywsyzyb.2023.05.015
[12] 李杰, 韩林, 王平, 等. 921名放射工作人员个人年有效剂量与外周血淋巴细胞微核率关系分析[J]. 中国辐射卫生,2019,28(5):487-490. DOI: 10.13491/j.issn.1004-714X.2019.05.004
Li J, Han L, Wang P, et al. Analysis of individual annual efficient dose and micronucleus results in peripheral blood lymphocytes of 921 radiation workers[J]. Chin J Radiol Health, 2019, 28(5): 487-490. DOI: 10.13491/j.issn.1004-714X.2019.05.004
[13] Deng J, Fan SN, Wang T, et al. Trends and distribution analysis of occupational exposure from medical practices in China (2010-2016)[J]. Health Phys, 2019, 117(6): 656-660. DOI: 10.1097/HP.0000000000001118
[14] Tapio S, Little MP, Kaiser JC, et al. Ionizing radiation-induced circulatory and metabolic diseases[J]. Environ Int, 2021, 146: 106235. DOI: 10.1016/j.envint.2020.106235
[15] 刘剑英, 尚伟华, 马得勋, 等. 长期低剂量核辐射接触人员机体相关指标变化[J]. 中国辐射卫生,2023,32(2):167-170. DOI: 10.13491/j.issn.1004-714X.2023.02.015
Liu JY, Shang WH, Ma DX, et al. Changes in body-related indices in people exposed to long-term low-dose nuclear radiation[J]. Chin J Radiol Health, 2023, 32(2): 167-170. DOI: 10.13491/j.issn.1004-714X.2023.02.015
[16] Mavragani IV, Nikitaki Z, Kalospyros SA, et al. Ionizing radiation and complex DNA damage: from prediction to detection challenges and biological significance[J]. Cancers, 2019, 11(11): 1789. DOI: 10.3390/cancers11111789
[17] Cwikel JG, Gidron Y, Quastel M. Low-dose environmental radiation, DNA damage, and cancer: the possible contribution of psychological factors[J]. Psychol Health Med, 2010, 15(1): 1-16. DOI: 10.1080/13548500903431493
[18] Nersesyan A, Fenech M, Bolognesi C, et al. Use of the lymphocyte cytokinesis-block micronucleus assay in occupational biomonitoring of genome damage caused by in vivo exposure to chemical genotoxins: past, present and future[J]. Mutat Res Rev Mutat Res, 2016, 770: 1-11. DOI: 10.1016/j.mrrev.2016.05.003
[19] Angelini S, Kumar R, Carbone F, et al. Micronuclei in humans induced by exposure to low level of ionizing radiation: influence of polymorphisms in DNA repair genes[J]. Mutat Res Fundam Mol Mech Mutagen, 2005, 570(1): 105-117. DOI: 10.1016/j.mrfmmm.2004.10.007
[20] Bouraoui S, Mougou S, Drira A, et al. A cytogenetic approach to the effects of low levels of ionizing radiation (IR) on the exposed Tunisian hospital workers[J]. Int J Occup Med Environ Health, 2013, 26(1): 144-154. DOI: 10.2478/s13382-013-0084-4
[21] Sari-Minodier I, Orsière T, Auquier P, et al. Cytogenetic monitoring by use of the micronucleus assay among hospital workers exposed to low doses of ionizing radiation[J]. Mutat Res Genet Toxicol Environ Mutagen, 2007, 629(2): 111-121. DOI: 10.1016/j.mrgentox.2007.01.009
[22] Fang LY, Li JQ, Li WG, et al. Assessment of genomic instability in medical workers exposed to chronic Low-Dose X-Rays in northern China[J]. Dose Response, 2019, 17(4): 1559325819891378. DOI: 10.1177/1559325819891378.
[23] 李丽梅, 高朝贤, 惠长野, 等. 深圳市2015—2020年放射工作人员上岗前职业健康检查染色体畸变率与微核率分析[J]. 国际放射医学核医学杂志,2022,46(8):471-477. DOI: 10.3760/cma.j.cn121381-202201015-00211
Li LM, Gao CX, Hui CY, et al. Analysis of chromosome aberration rate and micronucleus rate of pre-job occupational health examination of radiation workers in Shenzhen from 2015 to 2020[J]. Int J Radiat Med Nucl Med, 2022, 46(8): 471-477. DOI: 10.3760/cma.j.cn121381-202201015-00211
[24] Hopf NB, Danuser B, Bolognesi C, et al. Age related micronuclei frequency ranges in buccal and nasal cells in a healthy population[J]. Environ Res, 2020, 180: 108824. DOI: 10.1016/j.envres.2019.108824
[25] Cai TJ, Lu X, Tian XL, et al. Effects of age and gender on the baseline and 2 Gy 60Co γ-ray-induced nucleoplasmic bridges frequencies in the peripheral blood lymphocytes of Chinese population[J]. Mutat Res Genet Toxicol Environ Mutagen, 2018, 832-833: 29-34. DOI: 10.1016/j.mrgentox.2018.06.013
[26] Laffon B, Bonassi S, Costa S, et al. Genomic instability as a main driving factor of unsuccessful ageing: potential for translating the use of micronuclei into clinical practice[J]. Mutat Res Rev Mutat Res, 2021, 787: 108359. DOI: 10.1016/j.mrrev.2020.108359
[27] 杨永清, 董伯森, 赵芳, 等. 邯郸市541名放射工作人员淋巴细胞微核分析[J]. 职业与健康,2011,27(16):1816-1818. DOI: 10.13329/j.cnki.zyyjk.2011.16.024
Yang YQ, Dong BS, Zhao F, et al. Analysis on micronucleus of lymphocytes of 541 radiation workers in Handan city[J]. Occup Health, 2011, 27(16): 1816-1818. DOI: 10.13329/j.cnki.zyyjk.2011.16.024
[28] Azizova TV, Hamada N, Grigoryeva ES, et al. Risk of various types of cataracts in a cohort of Mayak workers following chronic occupational exposure to ionizing radiation[J]. Eur J Epidemiol, 2018, 33(12): 1193-1204. DOI: 10.1007/s10654-018-0450-4
[29] Tang FR, Loke WK, Khoo BC. Low-dose or low-dose-rate ionizing radiation-induced bioeffects in animal models[J]. J Radiat Res, 2017, 58(2): 165-182. DOI: 10.1093/jrr/rrw120
[30] 白玉书, 陈德清. 人类辐射细胞遗传学[M]. 北京: 人民卫生出版社, 2006.
Bai YS, Chen DQ. Human radiation cytogenetics[M] Beijing: People's Medical Publishing House, 2006.
|
|
|
|
