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  • 28 December 2012 Volume 21 Issue 4
      
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  • The Effects of Heat Shock Induction Expression of HSP70 on Hprt Genetic Mutation Induced by X-ray in Lymphocytes
    LIU Rui-fang, HONG Cheng-jiao, LI Yi-jie, ZHANG Bao-guo
    2012, 21(4): 385-387. https://doi.org/10.13491/j.cnki.issn.1004-714x.2012.04.078
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    Objective To explore the effects of heat shock pretreatment on hprt genetic mutation induced by X-ray in lymphocytes. Method The rat peripheral lymphocytes were cultured under 42℃ and 5% CO2 for 90min, then the cells were divided into 4 groups (control group, heat shock group, radiation group, heat shock + radiation group), each group including 6 parallel samples. And each group was fractionated into 2 parts, one part with 6-TG (final concentration was 0.1 mmol/L) and the other part without 6-TG. Each group of cells were irradiated by 0.5Gy、1.0 Gy、1.5 Gy、2.0 Gy、2.5 Gy、3.0 Gy X-ray and recovery cultured for 3h,6h,10h, and then polykaryocytes test was used to detect the hprt mutation in the rat peripheral lymphocytes. Results After exposure to different dose X-ray and recovery culture for 3h,6h,10h, the mutation rate of hprt in heat shock + radiation group was significantly decreased (P < 0.01) compared to radiation group. Conclusion Heat shock pretreatment has protection effects on hprt mutation induced by X-ray. HSP70 has more obvious protection effects in low dose radiation than high dose radiation on hprt mutation induced by X-ray (P < 0.01). There is negative correlation between protection effects and radiation doses.
  • The Position Error Analysis and Quality Control of Electron Portal Imaging Device (EPID) in Cervical Carcinoma Reverse Cancer Intensity Modulated Radiotherapy
    LIANG Guang-li, DU Wu, JIANG Sheng-peng, SUN Jian
    2012, 21(4): 387-389. https://doi.org/10.13491/j.cnki.issn.1004-714x.2012.04.071
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    Objective Through Electron Portal Imaging Device,EPID to detrerming and analysize the position error of intensity modulated radiation therapy and calculate the CTV extended PTV boundary size. Methods Ortholopic and lateral films were tahen before the accelerator for each patient once a week before treatment for verification. Position errors were calcal ated by camparing with DRR films in therapy planning systom. Results The PTV expansion throretical boundary was calculated cancer radiotherqpy intensity modulated radiation therapy. Conclusion Based on the position error analysis CTV expansion boundary provides theoretical basis for radiotherapy centre cervical cancer intensity modulated radiation therapy. Determination of CTV to PTV to expand outside edge, quality control measures help to decrease the boundary extension value decrease ceaselessly thas improve cervical cancer reverse IMRT treatment technique.
  • Research on Sensitive Indexes of Radiation Exposure Persons
    WANG Dong-ping, WANG Jun, LI Guang-wen, GAO Zong-ke, ZHAO Zeng-qiang, LIANG Zheng, WEI Chao-lang
    2012, 21(4): 389-390. https://doi.org/10.13491/j.cnki.issn.1004-714x.2012.04.023
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    Objective To observe and test sensitive indexes of nucleus radiation ray of 186 military exposure persons to explore the damage effect of nucleus radiation ray. Methods By means of case control analysis, sensitive indexes of nucleus radiation ray of 186 military exposure persons were observed. Results With the increasement of exposure length of nucleus radiation ray, both the rate of lymphocyte micronucleus and chromosome aberration in Blood will increase to some extent, but the total means values were still in ordinary range. When exposure length of nucleus radiation ray was over 10 years, rate of lymphocyte micronucleus and chromosome aberration in Blood had statistically significant difference compared with negative control group. Rate means values of urinary uranium and plutonium in urine had statistically no significant difference compared with negative control group. Rate of lens opacity gradually increase. Rate means values of lens opacity had statistically significant difference compared with negative control group. Conclusion There were certain nucleus radiation injury for military exposure persons, but the damage effect was in the acceptable range.
  • Preliminary Study of Reference Levels on Nuclear and Radiation Emergency Rescue
    TANG Mu-tao, HUANG Wei-chu, WANG You-jin, SUN Hua-bin, WANG Xiu-de, LI Qi-hui, ZHANG Hui-bin
    2012, 21(4): 391-393. https://doi.org/10.13491/j.cnki.issn.1004-714x.2012.04.024
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    Objective To discuss the influence of reference levels used to construct protection strategy in nuclear and radiation emergency recsue. Methods Protection strategies based on reference levels are constructed according to the newly published nuclear safety standards of IAEA and ICRP. Results The application of reference levels is more reasonable than the intervention levels proposed before from the established method to the indication quantity on dosimetry and the methods of optimization. Conclusions The application of reference levels have a far-reaching impact on making nuclear rescue standards and plans. It is suggested that the research on optimization of reference levels should be strengthened,and a unified method should be recommended.
  • Management Software for the Database of Gross α and Gross β in the Nationwide Drinking Water
    ZHANG Jing, JI Yan-qin, ZHOU Qiang, YIN Liang-liang, LI Wen-hong, XU Cui-hua, SU Xu
    2012, 21(4): 393-395. https://doi.org/10.13491/j.cnki.issn.1004-714x.2012.04.007
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    Objective A software was designed and developed for effective utilization sampling survey data of gross α and gross β in drinking water throughout China. Methods Survey data were picked up by using Microsoft Access data base. Management software of the data base was developed with Visual Basic 6.0 Enterprise Edition. Results Based on current status of the survey data, the database was established and the relevant help file was set up. Conclusions A convenient platform for the full use of the document of gross α and gross β in drinking water has been provided with the software in China.
  • Dose-Effect Relationship between Premature Chromosome Induced by CalyculinA and Low-dose Ionizing Radiation
    XIN Ya-zhou, SUN Hong-qiang, MA Ya, LU Feng, HOU Dian-jun, LI Jie-qing, LIU Wei
    2012, 21(4): 396-398. https://doi.org/10.13491/j.cnki.issn.1004-714x.2012.04.025
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    Objective To explore the feasibility of CalyculinA induced premature chromosome condensation to be used as a biodosimetry of low-dose ionizing radiation. Methods Peripheral blood was drawn from healthy adults and Anticoagulated with Heparin. a set of tubes was irradiated by X-ray. The irradiation dose included 0, 0.1,0.25,0.5,0.75,1.0 Gy. After irradiation, the whole blood was added to each RPMI1640 medium, All incubations were Cultured for up to 48h at 37℃ in an atmosphere of 5% CO2 in air. Colcemid was added 24hours after cultures start and Calyculin A was added for the last 2 hours to induce PCC. The chromosome spreads were prepared, stained with Giemsa and observed under a microscope. The dose-response relationship of the total aberration,fragment,dicentric + centric ring(dic + r) in the irradiated lymphocytes was examined. Results The PCC was successfully induced in lymphocytes with Calyculin A. The yield of chromosome aberrations increased with the dose going up. An aquadratic polynomial dose response model was fitted. Conclusion Premature chromosome condensation induced by Calyculin A can be used as a technology to estimate the radiation dosage of low-dose ionizing radiation, but still need more data to improve.
  • The Radioprotective Effect of Human Lactoferrin in Mice
    FENG Li, LI Fu-sheng, WEI Chao, ZHANG Yuan, QIAO Jian-wei, SHAO Chuan-jing
    2012, 21(4): 399-400. https://doi.org/10.13491/j.cnki.issn.1004-714x.2012.04.026
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    Objective To study the effect of human lactoferrin on the X-ray Radiation Injury in mice. Methods The mice were randomly divided into three groups:control group,radiation group and l1actoferrin group. To give mice 6.8 Gy, observe mice'30 d survival rate and to give mice 2 Gy,observe bone marrow micronucleus, nuclear cell count. Results All the mice of radiation group without Lactoferrin died in 25d after exposure. The group the 30-day livability is high,there was a significant difference in comparison between radiatin group without lactoferrin and groups with lactoferrin (P < 0.05). In the group without lactoferrin, the bone marrow karyocytes decreased significantly after radiation, and lf treatment groups (including prevention and emergency) are higher than pure radiation group, there was a significant difference in comparison between radiatin group without lactoferrin and groups with lactoferrin (P < 0.05). Simply after radiation for 3 days, mice micronucleus bone marrow cells shouwed a significantly higher rate, and the group without lactoferrin, it shouwed less than the group with lactoferrin, with significant difference (P < 0.05). Conclusion lactoferrin which is used in the prevention iron or emergency to has radioprotective effect.
  • The Influence of the Long Term Voyage on the Lymphocytes of Nuclear-Powered Submarine Crews
    SUN Hai-wen, MA Gui-xi, LI Jing, LV Xiao-ning, MENG Guo
    2012, 21(4): 401-402. https://doi.org/10.13491/j.cnki.issn.1004-714x.2012.04.079
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    Objective To study the influence of long term voyage on the number and activity of the nuclear-powered submarine crew's lymphocytes. Methods Before and after long term voyage, the total number lymphecytes of peripheral blood were measured; pured lymphecytes were planted to count the number of T lymphocyte colonies; and the apoptosis rate T lymphecyte was measured. Results After long term voyage, the total amount of lymphecyte is increased; the T lymphocyte make much more colonies after planting; and the apoptosis rate of T lymphecyte is raised. Conclusion Long term voyage in nuclear-powered submarine can affect the account of peripheral blood lymphocyte and the activity of T lymphocyte, The cumulative effect of micro-radiation in cabin can affect the immune function of the crew, more protection need to be strengthened to enhance the health.
  • The Monitoring of Radiation dose to Patients in Cardiovascular Interventional Procedures
    DING Yan-qiu, ZHANG Ji-mian, JIAO Ling, WU Quan, ZHANG Wen-yi, KOU Ming-ying, ZHANG Xiao-dong, FENG Xin, XU Ming-fa, ZHANG Liang-an
    2012, 21(4): 403-405. https://doi.org/10.13491/j.cnki.issn.1004-714x.2012.04.027
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    Objective To monitor and analyze the radiation dose to patients in cardiovascular interventional procedures and the radiation dose-related indicators, in order to provide a basis data for improving radiation protection of patients. Methods The clinical data of 60 cases of complete cardiovascular interventional procedures were collected; including coronary angiography (CA) and percutaneous transluminal coronary angioplasty (PTCA). The patient-received radiation doses and other related factors were studied. TLD personal dosimeter radiation field matrix method was used to measure fluorescence time, the entrance skin dose (ESD), the peak skin dose (PSD), dosearea product (DAP) and other indicators. Results The range of fluoroscopy time was 1.0~44.7min. The range of average entrance skin dose (ESD) was 2.6~283.5mGy. The range of peak skin dose (PSD) was 8.6~1156.9 mGy. The range of dose-area plus is 1.5~170.1 Gy·cm2. The effective dose is 0.2~23.8 mSv. The dose along irradiation field is 0.1~371.6 mGy. The fluorescence time, entrance skin dose (ESD),peak skin dose (PSD) of the group CA + PTCA are greater than the group CA and the difference has statistical significance(P < 0.05). Comparing different puncture methods, the fluorescence time, ESD, PSD of the group CA + PTCA are less difference with the group CA. Conclusion The administer department should pay more attention to the peak skin dose of the patient received in cardiovascular interventional radiological operation.