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  • 28 April 2013 Volume 22 Issue 2
      
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  • Radialion Induced BLAP75 Phosphorylation and Its Nuclear Localization Change
    XU Chang, CAO Jia, DU Li-qing, WANG Yan, LIU Qiang, FAN Sai-jun, FAN Fei-yue
    2013, 22(2): 129-131. https://doi.org/10.13491/j.cnki.issn.1004-714x.2013.02.001
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    Objective To study how BLAP75 responses to ultraviolet radiation and ionizing radiation. Methods Using Western blot and indirect immunofluorescence to detect the effects of ultraviolet and ionizing radiation on BLAP75. The type of post-translational modification of BLAP75 was determined by λ-Protein Phosphatase assay. Results After cells were treated with UV or IR, BLAP75 protein got post-translational modification, which was partially inhibited by caffeine. In response to IR, BLAP75 was recruited to nuclear foci. Conclusion UV and IR results in the phosphorylation of BLAP75 and ATM or ATR is responsible for part of it. BLAP75 is possible to participate in DNA repair on nuclear foci.
  • A New Software Developed for Internal Dose Assessments of Patients Undergone Diagnostic Nuclear Medicine Procedures
    YI Yan-ling, ZHAO Chao, YANG Li, ZHUO Wei-hai
    2013, 22(2): 132-134. https://doi.org/10.13491/j.cnki.issn.1004-714x.2013.02.015
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    Objective To cope with the newest tissue weighting factors recommended by ICRP and develop a set of software for convenient estimation of the organ dose and effective dose of patients inclinical nuclear medicine. Methods Relevant electronic databases were built based on the dose conversion coefficients recommended byICRP. The organ doses were estimated using the S value method, and the effective doses were evaluated by using the newest tissue weighting factors. The software was developed by using C++ programming language. Results The software can be used to estimate the absorbed doses of 27 organs/tissues and the effective doses for patients in clinical nuclear medicine applications involving a total of 47 kinds of radionuclides and 268 chemical compounds. The variance of estimated effective dose for patients undergoing diagnostics with 99mTc、18F and 131I are 20%, 8% and 32% by using different versions of tissue weight factors. Conclusions The database established in this study contains a large number of radionuclides and their compounds. It can be applied for simple estimations of the organ dose and effective dose of patients in clinical nuclear medicine procedures.
  • Study on MiR-200 Family Expression in the Stages of Transformation of Human Bronchial Epithelial Cells Induced by α-Particles
    GAO Gang, YANG Ying-jie, LIU Jian-xiang, TIAN Mei, SU Xu
    2013, 22(2): 135-137. https://doi.org/10.13491/j.cnki.issn.1004-714x.2013.02.002
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    Objective To investigate miR-200 family expression in the stages of transformation of human bronchial epithelial cells induced by α-particles. Methods Real Time PCR was used to detect miR-200 family and ZEB family and epithelial mesenchymal transition marker expression in transformed cells induced by α-particles. Results The downregulation of mature miR-200a, miR-200b, miR-200c and miR-141 expression in RHT35 cells was detected by Real Time PCR compared with R15H20 and BEP2D cells. The mRNA level of ZEB1 and ZEB2 in RHT35 cells upregulate compared with R15H20 and BEP2D cells, P < 0.01. The mRNA level of E-cadherin in RHT35 cells downregulate and N-cadherin upregulate compared with R15H20 and BEP2D cells, P < 0.01. Conclusion MiR-200 family are differentially expressed in the stages of transformation of human bronchial epithelial cells induced by α particles.
  • Estimation of Internal Dose from Primordial Radionuclides for Chinese Adult Men By Using Indirect Approac
    WU Quan, LIU Qing-fen, FAN Ti-qiang, ZHU Hong-da
    2013, 22(2): 138-140,144. https://doi.org/10.13491/j.cnki.issn.1004-714x.2013.02.016
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    Objective To estimate internal dose from primordial radionuclides for Chinese adult men in four areas by using indirect appoach. Methods With duplicate portion study, mixed diet samples were collected from adult man in the city of Tianjin,Chengdu,Zhenjiang and Taiyuan,inhalabe particle samples in air were also collected at the same time. The concentrations of rubidium,uranium, thorium in these samples were determined by Inductively coupled plasma mass spectrometry (ICP-MS),and the intake of them were calculated. Results The internal dose from primordial radionuclides for Chinese adult men in four areas were obtained combined with the literature. Conclusions The estimation values are similar to previous estimations.
  • Setting-up of Radiation-Induced Kidney Injury in Rat Model and Evaluation of Image Manifestations
    MAO Yu, CAO Yong-zhen, ZHOU Yan, WEI Jian, LI Su-fen, ZHANG Wen-xue, GENG Kai, ZHANG Yin-ling
    2013, 22(2): 141-144. https://doi.org/10.13491/j.cnki.issn.1004-714x.2013.02.046
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    Objective To study the setting-up methods of radiation-induced kidney injury in rat model, the pathological changes of in the early phase of radiation-induced kidney injury, and assess the diagnostic value of CT and MRI in the early phase of radiation-induced kidney injury. Methods Forty-eight Sprague Dawley rats were randomly divided into one normal control group and two experimental groups, and the experimental groups received a single dose of 20 Gy to the left kidney. Images of CT and MRI were obtained and pathological observation on the kidneys at 4, 12, 24, 36 weeks. Results CT、MRI findings including kidney atrophy and the thin of kidney parenchyma and the loss of corticomedullary differentiation were observed at the scheduled study. Radiation nephropathy was detected earlier with MRI than CT, and the sensitivity of MRI was higher than that of CT (P < 0.05). Pathological observation showed the morphological changes at 4 weeks after irradiation,including glomerular and tubular injury. An improvement in glomerular and tubular lesions was observed by 12、24、36 weeks after irradiation. Conclusion The radiation-induced kindey injury model can be setting up with the exposure of a single dose of 15 Gy radiation to the unilateral kidney of sprague dawley rats and morphological changes was observed. Early diagnosis of radiation-induced kidney injury can be performed with MRI scanning better than CT.
  • Determination of Pyrethroids and its Metabolites in Human Urine by High-Performance Liquid Chromatography
    MENG Xiao, SHAO Hua, ZHANG Zhi-hu
    2013, 22(2): 215-217. https://doi.org/10.13491/j.cnki.issn.1004-714x.2013.02.034
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    Objective To develop a quantitative method for determination of pyrethroid insecticide (fenvalerate) and its metabolites (3-PBA) in human urine by high-performance liquid chromatography. Methods After acidification and C18 SEP-PAK solid phase extraction,the urine samples were analyzed by Zorbox SB C18 separation and DAD detector. Results A good liner relationship was obtained and the correlation coefficient were greater than 0.996. Relative standard deviation(RSD) and recoveries of fenvalerate and 3-PBA were both less than 10.0% and greater than 75%. Limit of detection of fenvalerate and 3-PBA were 0.78 μg/ml and 1.93 μg/ml respectively. Conclusion As it is convenient, low cost and reproducible, the method can meet biomonitoring requirements for general and special populations.