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  • 28 September 2010 Volume 19 Issue 3
      
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  • Study on the Therapeutic Role of Transplantation of Human Umbilical Cord Mesenchymal Stem Cells in NOD-SCID Mouse Model of Gastrointestinal Radiation Damage
    CAO Xiao-cang, WANG Bang-mao, ZHAO Hui, et al
    2010, 19(3): 257-261. https://doi.org/10.13491/j.cnki.issn.1004-714x.2010.03.056
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    Objective To explore the therapeutic role of transplantation of human umbilical cord mesenchymal stem cells (MSC) in NOD-SCID mouse model of gastrointestinal radiation damage and search for the reliable donator cell resource for the treatment of acute enter radiation disease.Methods To observe the potential of multi-differentiation of huma umbilical cord MSC in vitro, transplant human umbilical cord MSC which were labeled with CM-DiI to the mouse model and study not only the pathological changed of entero-mucosa using histochemical stain but also the location of MSC in the entero-mucosa of mouse model using confocal microscopy.Results The lesions in the mice treated with MSC were improved obviously compared with that of control mice.Many CM-DiI+ MSCs derived cells were distributed in intestinal mucosa and submucosa of the recipient mice, which indicated that MSCs-derived cells were involved in regeneration of the gastrointestinal radiation damage.Conclusion The data indicate that systemically administrated MSCs maybe accelerated tissue repair in mouse model and it is hopeful in the treatment of human acute enter radiation sickness in the future.
  • The Effect of Radiation on Hematopoiesis in Three Kinds of Mouse
    LI De-guan, WANG Yue-ying, WU Hong-ying, et al
    2010, 19(3): 261-262. https://doi.org/10.13491/j.cnki.issn.1004-714x.2010.03.057
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    Objective To study the effect of radiation on hematopoiesis in three kinds of mouse.Methods The experiment was designed to study the effects of radiation onWBC、BMC and DNA damage on the different time in three kinds of mice after 137Cs ray irradiated.Results The number of WBC, BMC and DNA reached the lowest level on the second day after radiation.The ninth day was a quick recovery stage.On the 21th day, the IRM-2 mouse's WBC BMC and DNA had recovered to 60.7%、82.1%、80.1%, ICR recovered to 60.7%、82.1%、80.1% and 615 mouse recovered to 50.8%、75.4% compared with their normal level.There were significant difference between IRM-2 and ICR or 615 mouse (P<0.01)Conclusions IRM-2 mouse resume quicklier than ICR and 615 mouse on hematopoiesis after radiation.
  • The Effects of Ionizing Radiation Combined with Autophagy Inducers or Inhibitors on Human Cervical Cancer Hela Cells
    QI Ya-li, WANG Jun, LI Yan, et al
    2010, 19(3): 263-265. https://doi.org/10.13491/j.cnki.issn.1004-714x.2010.03.050
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    Objective To detect the effects of ionizing radiation combined with autophagy inhibitors and inducers on the proliferation of human cervical cancer cell line.Methods MTT and flowcytometry (FCM) were used to detect the surviving and proliferation of human cervical cancer cells, and analysis of the relationship of dose-effect and time-effect was made.Results With the increase of irradiation doses (2, 4, 6, 8 and 10 Gy) and the elongation of irradiation time (24, 48 and 72 h), the inhibiting effect of ionizing radiation on the proliferation of human cervical cancer cells increased (P< 0.05 or P< 0.01).The inhibiting effect of 6 Gy combined with autophagy inducer rapamycin on the proliferation of Hela cells weakened (P< 0.05).The inhibiting effects of 6 Gy combined with autophagy inhibitor 3-MA on the cell proliferation were higher than those in 6 Gy group (P< 0.05).Conclusion Ionizing radiation combined with autophagy inducers can inhibit apoptosis in Hela cells, while the ionizing radiation combined with autophagy inhibitors can promote their apoptosis.
  • The Effect of LPS on Cytokines IL-10、IL-6 and TNF-α Level in Radiation-exposed Mice
    LU Lu, WANG Yue-ying, LI De-guan, et al
    2010, 19(3): 265-266. https://doi.org/10.13491/j.cnki.issn.1004-714x.2010.03.058
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    Objective To investigate the effects of LPS (lipopolysaccharide, LPS) on mice wholy exposed to the different single dose of 137Csγ-radiation.Methods Enzyme-linked Immunoassay (ELISA) was used to detect cytokines IL-10、IL-6 and TNF-α level at different doses and time.Results The level of IL-10、IL-6 and TNF-α in blood serum changed little among different radiation groups, but increased after LPS injection in the same radiation groups (P<0.05).Conclusion The results indicated that exposure to radiation could influence cytokines IL-10、IL-6 and TNF-α level of mice and immune system in response to the challenge with LPS.
  • The Radiation of Handportable Mobilephone Accelerates the Formation of Foam Cells Induced by Oxidized Low Density Lipoprotein in RAW 264.7 Macrophages
    WANG Ru-qiong, ZHOU Shou-hong
    2010, 19(3): 267-269. https://doi.org/10.13491/j.cnki.issn.1004-714x.2010.03.045
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    Objective To explore the effects of radiation of handportable mobilephone on the formation of foam cells induced by oxidized low density lipoprotein in RAW 264.7 macrophages.Methods RAW 264.7 macrophages were divided randomly into three groups:control group, oxidized low density lipoprotein (Ox-LDL) group, Ox-LDL + radiation group.Macrophages were incubated with 50 mg/l Ox-LDL for 48 h to induce foam cell.The radiation of handportable mobilephone was administered every 2 h from 8:00 AM to 20:00 PM.Results Compared with the Ox-LDL group, the levels of intracellular total cholesterol, free cholesterol and cholesteryl ester were increased significantly and there were more lipid droplets in cells in Ox-LDL + radiation group.Condusion The radiation of handportable mobilephone accelerates the formation of foam cells induced by oxidized low density lipoprotein in RAW 264.7 macrophages.
  • Study the Radiosensitive Effect of ELAC2 by Using Colony Formation Assay and Western Blot Assay
    NING Ping, JIAO Yang, HU Xu-dong, et al
    2010, 19(3): 269-271. https://doi.org/10.13491/j.cnki.issn.1004-714x.2010.03.001
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    Objective To study the effects of ELAC2 gene on the human breast cancer cell MCF-7's radiosensitivity.Methods ELAC2 gene was transfected into MCF-7 via Lipofectamine transfection assay, while MCF-7 cells transfected with/without empty vector MCF-7/pcDNA3 were also established as negative control.The mRNA expression of ELAC2 gene was detected by RT-PCR assay.The colony formation assay was performed to test the radiosensitivity.The protein expression of ELAC2, FEN-1, MGMT, MRE11and Ku-70 were detected by western blot assay.Results Overexpressed ELAC2 level was both observed at mRNA and protein level of the cells transfected with pcDNA3.0/ELAC2.After 0, 0.5, 1, 2, 4, 6Gy X-ray exposure, the radiation dose-response curve shifted to the left when ELAC2 overexpressed, and ELAC2 enhanced the radiosensitivity of MCF-7 cells.After 5Gy X-ray irradiation, no significant difference of MGMT and MRE11 protein expression levels were detected between ELAC2 overexpressed MCF-7 cells and control cells.While FEN-1 protein and Ku-70 protein expression levels were reduced significantly in MCF-7 cells with high level ELAC2.Conclusion ELAC2 gene could radiosensitize MCF-7 cells to X-ray irradiation, probably by the mechanisms of reducing the FEN-1 protein and Ku-70 protein expression.
  • Radiation Levels and Countermeasure Research on Radiological Protection in Tin Mine in China
    YANG Feng-fang, YUAN Yong-ling
    2010, 19(3): 272-274,276. https://doi.org/10.13491/j.cnki.issn.1004-714x.2010.03.019
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    Objective To study the distribution of radiation level in our nation's tin mine, therefore comes up with proposals on radiological protection and provide scientific evidence on how to protect lives and health of worker underground in tin mine.Methods To get the radiation level of the underground workplaces in tin mine by analysis of research papers and the measuring results from on-scene investigations.Results Majority of the absorbed dose rates of γ radiation in the air of underground workplaces in tin mine falls within the range of radiation levels of normal background.Earlier, the typical values of radon concentration and potential alpha energy concentration of radon daughters in the air of underground workplaces in tin mine are 3.12 kBq/m3 and 5.61μJ/m3 respectively.Now, radon concentration and potential alpha energy concentration of radon daughters in the air of underground workplaces in majority of tin mine are lower than 1 000Bq/m3 and 3.57μJ/m3.Conclusion For these tin mine workers with an average of annual effective dose greater than 1mSv or these of their specific activity for natural uranium in materials are greater than 1Bq/g need to have regulation of radiological protection.The control limits for the radon concentration and the potential alpha energy concentration of radon daughters and the absorbed dose rates of γ radiation in the air of underground workplaces in tin mine are 1 000Bq/m3, 3.57μJ/m3 and 1μGy/h respectively.The administrative individual dose for workers working under the ground of tin mine is 10mSv/a.If a worker's total annual effective dose is greater than 10mSv, he/she should be considered as radioactive worker.
  • The Preliminary Study on Effects of Behavior on Mice by Magnetic Free Fields Space
    WANG-Qian, SUN Hua-bin, WANG Xiu-de, et al
    2010, 19(3): 275-276. https://doi.org/10.13491/j.cnki.issn.1004-714x.2010.03.046
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    Objective To study the effects of behavior on mice by magnetic free fields space (MFFS).Methods The environment of MFFS was simulated by grade C magnetic shield.7 Balbc/c mice were put into both the MFFS and normal environment in sequence.The indexes of behavior were examined by UGO-BASILE-7431 ACTIVITY CAGE in the daytime and nighttime, and taken statistics by SPSS (copyright 11.0).Results Compared with the normal, statistically significant differences were not observed in the values of horizontal-activities in MFFS environment.But, the values of vertical-activities obviously increased;The values shows no differences between the daytime and nighttime.Conclusion Significant changes of some behavior indexes can be observed in MFFS environment, such as fidget, anxiety, etc.Geomagnetic environment is closely linked to the biological activities.
  • Estimation of the Protection Distance in Mobile Gamma-ray Radiography
    WEI Zheng, DIAO Duan-yang, ZHANG Ping
    2010, 19(3): 277-278. https://doi.org/10.13491/j.cnki.issn.1004-714x.2010.03.002
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    Objective To discuss the protection distance of unshielded γ ray in industrial radiography in open areas, and help the radiation workers to divide the boundary of controlled area and supervised area.Methods The distance was estimated by using the point-source formula and considering the absorption of air on different energy γ-ray.It's in line with the dose-rate limit of the control area and supervised area boundary.Rusults The controlled area and supervised area distance of commonly used radioactive sources 60Co, 192Ir and 75Se with different activity was given.Conclusion The distance of controlled area was reduced about 10% to 20% while considering the air absorption, the supervised area was reduced more than 20%.The radiation workers can use it to ensure the barriers fast and accurate.
  • Correlations Between Exposure Conditions and Dosimetric Parameters for Digital Radiography(DR)
    QU Liang-yong, GAO Lin-feng, YAO Jie, et al
    2010, 19(3): 279-281. https://doi.org/10.13491/j.cnki.issn.1004-714x.2010.03.020
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    Objective To study correlations between exposure conditions and dosimetric parameters for digital radiography (DR).Methods Quality assurance (QA) was performed with BARRACUDA system to make sure DR x-ray machine was in good condition.On condition that testing phantom was used, dosimetric parameters, such as dose area product (DAP), output of x-ray machine, entrance surface dose (ESD) were recorded while changing exposure conditions including kV, mAs and filter.Correlations between exposure conditions and dosimetric parameters were analyzed.Rusults DAP reduced from 173.80 mGy·cm2 to 33.39 mGy·cm2, output of x-ray machine increased from 0.010Gy/(A·s) to 0.032 Gy/(A·s), ESD reduced from 0.74 mGy to 0.17mGy, when kV raised steadily from 60 to 120.At 70kV, 90 kV and 110 kV, DAP and ESD increased steadily in same trend as mAs.The decrease extent of DAP and ESD was distinctly less when 2.5 mmAl+1mm Cu or 3.3mm Al+1mm Cu were used as total filter, comparing to 3.3 mmAl or 4.1 mmAl.For certain filter, there were significantly negative correlation (P<0.05) among DAP, ESD and kV, and there were significantly positive correlation (P<0.05) among DAP, ESD and mAs.Output of x-ray machine was correlated positively negatively with kV (P<0.05), negatively with mAs (P<0.05).The correlations were not affected by changing of kV or mAs.Conclusion For DR, the dose of examinees can be effectively reduced by properly raising kV or lowering mAs.