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  • 28 September 2009 Volume 18 Issue 3
      

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  • ZHANG Wei, WANG Li-li, ZHOU Ju-ying, et al
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    Objective To investigate the effect of magnesium sulfate(MgSO4) on the nerves function after hemisphere irradiation and explore possible mechanism of the effect.Methods Mature Sprague-Dawley rats were randomly divided into 3 groups:blank control group(n=26),experimental control group(n=32) and experimental group(n=32).To selecte rats randomly from the experimental control group and the experimental therapy group for 1/2 brain irradiation to a single-fraction maximal dose of 20Gy using 5MeV electrons.Magnesium sulfate was injected intraperitoneally into the rats that from the experimental group before and after irradiation for total seven times.The expression of c-fos gene was observed in the hippocampal formation of the rat brain with the immunofluorescence technique in 24h after irradiation.The learning and memory results in Y-maze test were detected 8 weeks after irradiation respectively with the other rats and then had the pathomorphology observation.Results Compared with the blank control group,the number of c-fos immunopositive cells in the hippocampal formation of experimental control group were increased markedly(P<0.01),and the learning and memory results of Y-maze test in the irradiation control group declined significantly(P<0.01).Compared with the irradiation control group,the experimental group's Fos immunopositive cells decreased significantly(P<0.05),and also the cognitive function of learning attempt times(at 4 and 8 weeks,P<0.01) and memory reappearance times(P<0.05) were significantly different from that in the irradiation control group.Conclusion Magnesium sulfate can promote the recovery of the rats' cognitive impairment in the early stage after irradiation,and may play a role of protecting hippocampus neurons by the way of decreasing the expression of Fos protin in hippocampal formation.
  • Guo Jian-ping, YANG Zhong-tian, ZHOU Chao-dong
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    Objective To study the radiation protection of hemopoietic system of oligo-chitosan for mice injured by 60CO irradiation.Methods Using 60Co γ-ray irradiated mice,the influence of Oligo-chitosan on 30 d survival rate,mitotic index of marrow,colony-forming unit-spleen,index of spleen and nucleated cell count in bone marrow of the irradiated mice were observed.Results The results show that Oligo-chitosan could increase their 30 d survival rate;increase significantly MI and nucleated cell count in bone marrow at middle and high doses and CFU-S at high dose.Conclusion Oligo-chitosan could reduce the injury by radiation and protect hemopoietic system of radiated mice.
  • WENG Wan-Weng, XU Yu-Jie, WANG Zong-Zhan, et al
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    Objective To used to profile changes in gene expression of KB cell after exposed to ionizing radiation.Melhodds ① Inhibitory effect of different dose of radiation on KB cell line growth was analyzed using colony formation test;② Oligo microarray was used to screen the genes showing altered expression compared with control group.Results Data analysis showed 749 genes have been changed after exposed to ionizing radiation,including 269 up-regulated and 480 down-regulated.These genes can be classified into clusters of cell division,nucleic acid synthesis and repair,nucleic acid binding and transcription and apoptosis etc.Conclusion It is suggested that a variety of regulatory pathways in KB cell line were affected by irradiation.And research about the change of the gene express profile could better clarify the mechanism of radiation sensitivity and insensitivity.
  • WANG Yong, WANG Yan, DU Li-qing, et al
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    Objective To investigate the possible radiosensitive effect of human Lactotransferrin(hLF) high expression in lung cancer cell,we hereby constructed and transfected a recombinant vector pBC1 containing exogenous hLF gene.Methods Firstly the recombinant plasmid pBC1-hLF was transferred into H460 and the hLF expression was evaluated by western blotting.Then we test the cell viability,apoptosis and clone formation after radiation along with the standard hLF treatment control.Results The results showed that the hLF gene had been successfully transfect into H460 cells and hLF high expression can reduce the clone formation after radiation(P<0.01),and inhibit the cell proliferation with induced apoptosis(P<0.01).And the sensitization ratio of hLF treated and pBC1-hLF transfected were 1.29,1.59.Conclusion Our primary data shows that hLF high expression can radio sensitize the H460 cell in vitro.
  • WANG Yan-ming
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    Objective To explore the micro-changes in latent period of radiation brain injury and the value on quantitative analysis with diffusion weighted imaging.Methods Animal model:selecting 25 healthy hybrid dogs,to radiate tempus dextrum of the dog brain with γ-ray 70Gy dose one time.The dogs brain before and at 2,4,8,10 and 12th week after irradiation.ADC values of alba and ectocinerea were measured respectively.The histological changes were observed under light and electron microscope.Reasults The alba,ectocinerea and whole brain ADC value of healthy dog brain are(71.89±(4.97) ×10-5 mm2/s,(69.77±4.33) ×10-5 mm2/s and(70.83±4.74) ×10-5 mm2/s.ADC values decreased obviously after irradiation.To compare with before irradiation,the variance have statistical significance,P<0.05.Conclusion After radiation,dog brain existed micro-changes injury.DWI is a most sensitive functional imaging technique in detecting hydrone diffusion capability changes.It can discover and quantitatively analyze the micro-changes preceding anatomy imageology by measuring the irradiated tissue ADC value.
  • LI Lin-lin, FENG Li-yun, YANG Yan-yan, et al
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    Objective To explore interaction effect between stress reaction and job burnout,personality,self-esteem in radiological medical personnel with path analysis.Methods 728 radiological medical personnels were investigated with Maslach burnout Inventory-General Survey(MBI-GS),Chinese Perceived Stress Scale(CPSS),Eysenck Personality Questionnaire(EPQ) and Self-esteem Scale.Results Multiple regression and path analysis revealed that there were statistically significant relation between stress reaction and job burnout,Personality and self-esteem.Conclusion Psychological stress is a complicated and multiple interaction of psychological stress related factors.
  • ZHANG Shu-xu, YU Hui, WANG Shi-qin, et al
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    Objective To investigate the accuracy,reliability and convenience of 2-D ionization chamber array MatriXX system in daily quality control of radiation therapy.Methods The percent depth dose(PDD),tissue maximum ratio(TMR),off-axis ratio profile,penumbra and field width of Varian 23EX linac were measured by MatriXX system combined with solid water phantom and compared with the data acquired by RFA300 three-dimensional water phantom.The dose distributions of static and dynamic intensity modulated radiation therapy(IMRT) plans created by Pinnac3 V8.0m three-dimensional treatment planning system(3D-TPS) were validated by MatriXX combined with the dose phantom made in home.Results The PDD and TMR of 6MV X-ray measured by MatriXX system were parallel to the curves acquired by RFA300,and the PDD curve of 15MV X-ray measured by MatriXX was nearly identic to that measured by RFA300.The values of penumbra measured by MatriXX were two or three times larger than those measured by RFA300,and when the field size was smaller than 3cm×3cm the penumbra of it could not be automatically calculated out by the MatriXX system.The characteristic information of field,such as symmetry and flatness and field width,can be acquired at once in a single sampling,which can be completed in less than 20mS.The qualitative and quantificational verification of 2-D dose distribution of IMRT plans can be quickly implemented by using MatriXX system.Conclusions The MatriXX system is very accurate and convenient and effective in quality control of clinical radiotherapy,and it's a perfect tool for the quality control procedure of radiation therapy.
  • DING Hao, WANG Sheng-zi, WANG Shu-yi, et al
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    Objective To observe the differences of the radiation-induced peripheral blood T cell and its subgroup changes between SPF and CV rats after nasopharyngeal radiation with gradient doses and explore the microflora factors in the pathogenesis of abnormal radiation-induced immunity status.Methods 8 from each SPF and CV rats were chosen for oropharyngeal bacteria cultivation and determination and the spleen organ coefficients.The rest were irradiated with 6MX linear accelerator in the nasopharyngeal fields at dose of 0、10、20、30Gy,5 in each group.24~36h later,blood T lymphocytes and their subgroups were detected by FCM.Results The bacteria of CV rats were pathogen mostly and the one from SPF rats was Proteus mirabilis uniquely.Spleen organ coefficients between two groups showed no statistical defference.CD3+、CD4+ lymphocytes and the ratio of CD4+/CD8+ of CV rats decreased dramatically after radiation is in close relation with radiation doses while The CD8+ lymphocyte increased a bit.The CD3+、CD4+、CD8+ lymphocytes and the ratio of CD4+/CD8+ of SPF rats remained in a stable level.Conclusions There exists the difference of radiation-induced injuries of immune system in relation with different microflora status.Micro-flora plays an important role in the radiation-induced immune system abnormity.
  • LI Jie, LI Jian, QI Guo-hai, et al
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    Objective To measure the dosimetry of Total Skin Electron Irradiation.Methods Varian23EX linear accelerator was used to produce electron.Dosimetric characteristics in SSD 400cm were measured with parallel-plate chamber,film,TLD,by using the six-dual fields technique.Results A uniformity of ±5% of the treatment plane is achievable.In single field,the skin dose was more than 80%,depth of the max dose was 0.6cm,depth of 80% and 50% dose were 1.10cm and 1.42cm.For six-dual fields,depth of the max dose was nearly on the skin,depth of 80% and 50% dose were 0.40cm and 1.00cm.The X-ray contamination was less than 2.2%.Multiplication factor was 3.05.Conclusion The dosimetric characteristics in TSET were much different from the general single beam.The measurement before the treatment is necessary.
  • SU Feng, HUA Wei, ZHENG Chuan-cheng, et al
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    Objective To ensure the health of staff engaged in tritium,the purpose of experiment is to find out a fast,convenient and reliable sample preparation and measurement methods for such routine monitoring.Methods We use the conventional distillation decolorization and non-decoloration quenching correction methods dealing with urine sample,and then carried out the urine sample liquid scintillation measurements,statistical analysis between the two measurements.Results By using above two different methods of sample pretreatment,the results that we measure tritium in urine sample are not obviously differet in comparison.Conclusion The above two different methods can be used for nuclear facilities staff and staff related to conventional tritium detection.However,non-decoloration quenching correction method is simpler and less in time and manpower than the conventional distillation method in operation.It is suitable for a large number of samples prepared,measured,and analyzed in a short period of time.
  • XIE Tian-hao, FAN Yi-jun, YE wan-zhong, et al
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    Objective To differentiate vascular parkisonism(VP) from Parkison's disease(PD) with 99mTc-TRODAT-1 brain SPECT semiquantitative analysis.Methods 11 VP patients,26 PD patients and 12 healthy(control) individuals were studied.A 740 MBq(20mCi) dose of 99mTc-TRODAT-1 was injected intravenously.After 3-4 hr brain SPECT imaging and semiquantitative analysis were acquired.The ratio of specific striatal 99mTc-TRODAT-1 binding was calculated by dividing the specific striatal uptake by the occipital binding.Results After a ROI semiquantitative analysis of SPECT imaging from Vp patients,PD patients and healthy controls were performed to abtain ratio striatal uptake to occipital binding,the specific binding in the VP patients was slightly lower than but not statistically different from that of the healthy controls in striatal areas.A significant decrease in striatal uptake of 99mTc-TRODAT-1(P<0.01) was found in PD patients.There was significant bilateral striatal asymmetry in PD patients but not in VP patients(P<0.01).An age-related reduction of the S/O ratios was observed in both the VP patients and the healthy controls(P<0.05) but not in the PD patients.Conclusion 99mTc-TRODAT-1 brain semiquantitative analysis is a reliable method to giagnosis Parkinson's disease early and to differentiate VP from Parkison's disease.
  • YE Yin-tao, XU Wen-qing, SHEN Xiu, et al
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    Objective To investigate the radiosensitizing effects of dihydrotanshinone I on H22 hepatoma-bearing mice.Methods To establish the mouse hepatoma H22 models which were divided into blank control group,pure radiation group,positive control group,dihydrotanshinone I(high,medium and low) groups.After irradiated,observe the growth of tumor's conditions,record epigenetic tumor irradiation time,calculated the delay time of tumor growth and enhancement factor(EF).After 22 days,the mice were killed,tumors stripped and the inhibition rate calculate.Results Dihydrotanshinone I(high,medium and low) groups inhibited the tumor growth better than the blank group,and dihydrotanshinone I(medium and high) group had the significant radiosensitizing effect.The enhancement factor Was 1.43,1.66,respectively.Conclusion Dihydrotanshinone I can inhibite the tumor growth and has the radiosensitizing effects on H22 hepatoma-bearing mice.
  • YANG Fei, HUANG Zhong-hang, LU Xiao-qing, et al
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    Objective To explore the relationship between long-time exposure to low-dose ionization radiation and health effects.Methods 1052 occupational subjects exposed to ionization radiation in Chengdu city were recruited in monitoring cohort in 2007,including 785 men(74.62%) and 267 women(25.38%).Individual exposure dose were monitored by Thermoluminescent Measurement.Health effects include blood routine examination,Chromosomal aberration,eye lens test,etc.Variance Analysis(ANOVA),χ2 Test and Univariate Procedure of General Liner Model(Covariance Analysis) were implemented to test the difference among subgroups with SPSS 13.0 software.Results Annual average of exposure dose of male and female were(0.76±0.65) mSv and(0.75±0.64) mSv.There is no statistical significant between sex subgroups(F=0.136,P=0.712).In females subgroup,the frequencies and ratios with low WBC,Low platelet,high RBC and high HGB were 30(11.2%),45(16.9%),4(1.5%) 和3(1.1%) respectively.And in male subgroup,frequencies and ratios of above index were 32(4.1%),147(18.7%),64(8.2%) and 115(14.6%) respectively.Except low platelet,the distribution differences of the rest three blood indexes between sex subgroups were statistically significant(χ2 test,P<0.01).Either in male or in female subgroups,no statistically significant difference of all health indexes(RBC,WBC,Platelet,HGB,and Chromosomal aberration) was observed in different radiation dose teams.Conclusion In this monitoring cohort,the health effects were related to hormesis and adaptive response as well as radiation damage accumulation effect of low-dose ionization radiation.Females were the sensitive group to suffer adverse effects,while blood indexes were the sensitive indexes for monitoring radiation exposure.
  • HU Li-feng, YING Zheng-ju, LIU Jiao
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    Objective To explore the evaluation method for CT number linearity by measuring CT numbers.Methods The model CTP401 of Catphan500 phanton was adopted to measure CT numbers of four objective substances,then CT number Linearity,contrast scales,and uncertainties were calculated.Results Statistically significant differences in CT numbers were found between the two reconstruction algorithms(P<0.001),The contrast scale of sharp reconstruction algorithm was 1.90×10-4 cm-1·Hu-1,and smooth reconstruction algorithm was 1.79×10-4 cm-1·Hu-1.Conclusions Qualified CT number linearity is important to image quality control in CT scanners,Reconstruction algorithms and uncertainties must be marked in test reports of CT number linearity.
  • MA Yu-qin, LIANG Hua-ju, XU Yao-ling, et al
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    Objective To investigate the rule of change of autoimmunity and the correlation with prognosis in patients with Graves' disease before and after being treated with 131I.Methods To measure Serum FT3,FT4,sTSH,TRAb,TGAb,TMAb,IL-2,IL-6 in 70 patients with Graves' disease at or before 3,6,9,12 months after being treated with 131I and 30 healthy persons are chosen as the control group.To observe the rule of change of the indexes and the correlation with prognosis(hyperthyroidism,cure,hypothyroidism) one year after treated with 131I.Results ① Serum indexes except sTSH in the patients before 131I treatment were significantly higher than those in controls(P<0.01).② The changing direction of TRAb was uptrend,and those of IL-2,IL-6,TGAb,TMAb were downtrend after treated with 131I.③ The prognosis was negative correlated with TRAb before treated with 131I(P=0.04) and IL-2 after treated(P=0.00),was positively correlated with TMAb after treated(P=0.02).Conclusion All of TRAb,TGAb,TMAb,IL-2,IL-6 are involved in auto-immunoreaction in Graves' disease,however the prognosis one year after 131I treatment was only correlated with TRAb before treatment with TMAb and IL-2 after treatment.