Archive

  • 28 June 2008 Volume 17 Issue 2
      
  • Select all
    |
  • The Effects of Electromagnetic Irradiation on Activation of Microglia and JAKs in Rat Hippocampus
    CHEN Chun-hai, YANG Xue-sen, HAO Yu-tong, et al
    2008, 17(2): 129-131. https://doi.org/10.13491/j.cnki.issn.1004-714x.2008.02.001
    Download PDF ( ) HTML   Knowledge map   Save
    Objective To determine the activation of microglia and the phosphorylation of Jaks, the upstream factors of JAK/STAT (janus activated kinase/signal transducers and activators of transcription)signaling pathway, after electromagnetic irradiation.Methods Rats were irradiated by 90 mW/cm2 EMF for 20 min.The phosphorylation of Jaks was determined by western blot at different time after electromagnetic irradiation.The activation of microglia was determined by immunochemistry.Results GSA-IB4 was upregulated in microglia, which indicated microglia was activated after electromagnetic irradiation.The phosphorylation of Jak1, Jak2 and Jak3 in rat hippocampus was upregulated after electromagnetic irradiation.The phosphorylation of Jak1 was upregulated after microwave exposure and peaked at 12 h.Jak2 peaked at 0 h after electromagnetic irradiation and sustained in a high level.Jak3 was slightly affected by electromagnetic irradiation.All the three members of JAKs return to normal at 72 h after electromagnetic irradiation.Conclusion Microglia cells was activated after electromagnetic irradiation.The phosphorylation of Jaks was upregulated by electromagnetic irradiation.It suggested that JAK/STAT singnaling pathway was activated after electromagnetic irradiation, which indicated that JAK/STAT signaling pathway may participate in brain microglia activation induced by electromagnetic irradiation.
  • Existing Condition Assessment of Radiation Environment Quality in Dwellings
    XU Jia-ang, LI Fu-sheng, CHEN Ying-min, et al
    2008, 17(2): 132-134. https://doi.org/10.13491/j.cnki.issn.1004-714x.2008.02.002
    Download PDF ( ) HTML   Knowledge map   Save
    Objective To assess the radiactivity levels of Jinan dwellings and the indoor environment quality.Methods Using the self made Measurement Cups of Indoor Environmental Radioactivity Evaluation (MCIERE) (ZL 200620082698.7)to measure the gamma ray dose rate, 222Rn activity concentration, 220Rn activity concentration and EECTn.Results The geometric mean of 222Rn activity concentrations that showed a clear lognormal distribution tendency in 411 rooms of Jinan was 45 Bq·m-3, the range which was from 18 to 203 Bq·m-3.The geometric mean of 220Rn activity concentration that was also nearly lognormal distribution in 203 rooms of Jinan was 16 Bq·m-3and the range of the activity concentratons was from 1 to 167 Bq·m-3.The geometric mean of EECTn that was nearly lognormal distribution in 204 rooms of Jinan was 1.082 Bq·m-3, the range was from 0.015 to 10.230 Bq·m-3.The arithmetic mean of the gamma ray dose rate that was nearly Gaussian distribution in 412 rooms of Jinan was 0.106μSv·h-1, the concentration range from 0.041 to 0.167μSv·h-1.Conclusion The internal irradiation annual effective dose caused by 222Rn and its progeny, 220Rn and its progeny and of Jinan inhabitant was 1.189 mSv, the external irradiation annual effective dose caused by the gamma ray from the ground and buildings was 0.743 mSv.The mean total annual effective dose was 2.187 mSv and the highest annual effective dose level was above 5 mSv.
  • Study on the Influence of Tumor Cell and Radiation on KH-HL Mice
    SHEN Xiu, HONG Ge, LIU Pei-xun, et al
    2008, 17(2): 135-136. https://doi.org/10.13491/j.cnki.issn.1004-714x.2008.02.066
    Download PDF ( ) HTML   Knowledge map   Save
    Objective To observe the susceptibility to tumor cell of KH-HL mice and study the descent of immune function by 137Cs source γ rays to provide theoretical foundations for KM-HL mice use as experimental mice.Methods The mice sarcoma (S180), cervical cancer (U14), and liver cancer (H22)are transplanted on KM-HL mice and the growth of mice bearing tumor is observed, its immune function of KH-HL mice descends when irradiate with 137Cs source γ rays at 6.5Gy, Some immunization indexes such as blood, hematopoietic system, spleen index and thymus index are examined.Results Both the liquid tumor cell and the solid tumor of sarcoma (S180), cervical cancer (U14), and liver cancer (H22)transplants on KM-HL mice grow well.After the irradiation, all of the physiological indexes of KM-HL mice descend comparing with the control group, with statistical significance (P<0.01).Conclusion The inbred line KM-HL mice can be used as experimental model mice in the screening of antitumor and immunological function drugs.
  • The Effect of Environment Factors of Ladar Army on Neurobehavioral Function of Military Task Population
    DENG Zhao-hui, GONG Qian-fen, YU Zheng-ping, et al
    2008, 17(2): 136-137. https://doi.org/10.13491/j.cnki.issn.1004-714x.2008.02.003
    Download PDF ( ) HTML   Knowledge map   Save
    Objective To evaluate the effect of the electromagnetic irradiation of ladar army on neurobehavioral function of military task population.Methods 40 workers exposed to electromagnetic irradiation and 20 controls were investigated with questionnaire survey, profile of mood state and some other neurobehavioral function tests.Results Of all the rational symptoms, visual fatigue is ware obvious in the irradiation group and fatigue of POMS form of irradiation group have significant increased.The sum of the pursuit aiming test and the second self intercrossing test have obvious decreased.Conclusion The mood state, hand operation ability and work efficiency in occupational people are affected by electromagnetic irradiation.
  • Experimental Study of the Effects of Kuan Jin Teng on Hematopoietic Function in Radiation-injured Mice
    DUAN Wei, BI Liang-wen, LI Wen-hui
    2008, 17(2): 138-140. https://doi.org/10.13491/j.cnki.issn.1004-714x.2008.02.050
    Download PDF ( ) HTML   Knowledge map   Save
    Objective To study the effects of Kuan Jin Teng on hematopoietic function in radiation-injured mice.Methods In this experiment, 50 mice were randomly divided into five groups of normal control, irradiation (IR), high dosage, medium dosage and low dosage.The last three groups were given medicines after they were irradiated by 60Coγ.The others were given physiological brine.Peripheral blood routine were measured at 4h before irradiation, the 7th and 14th after irradiation.DNA content and quantity of nucleocyte in bone marrow, CFU-s in spleen were observed at the 14th day after irradiation.Results The results showed the protective action of Kuan Jin Teng on peripheral blood routine, DNA content and quantity of nucleocyte in bone marrow of radiation-damaged mice is obvious.There was significant difference between the irradiation and experimental groups (P<0.05).Conclusion Kuan Jin Teng can improve the quantities of peripheral blood routine and promote the recovery of the cells of building blood in bone marrow and it is effective in anti-irradiation.
  • The Study on the Items of Oxidation and Anti-oxidation in the Rats with Radiation Esophagitis Treated with Compound White Peony Root Oral Liquids
    SHEN Li, SHAN Bao-en, ZHANG Li, et al
    2008, 17(2): 141-144. https://doi.org/10.13491/j.cnki.issn.1004-714x.2008.02.051
    Download PDF ( ) HTML   Knowledge map   Save
    Objective To study the content of the hydroxyl radical (OH·)and NO, the activity of CAT and GSH-PX in rats' esophagus and NO level in serum from the Wistar rats radiated for esophagitis area treated with compound white peony root oral liquids (cWPROL).Methods 96 Wistar rats were divided into eight groups.Group 1, the normal group, were without any radiation and treatment;Group 2, radiation group 1, killed on the seventh day after being radiated by 43Gy 60Co-γ ray;Group 3, radiated group 2, killed on the fifteenth day after being radiated;Group 4 given cWPROL (normal dose)for prevention at the same time of radiation;Group 5 given cWPROL (high dose)for prevention at the same time of radiation;Group 6 were treated with cWPROL (normal dose)from the seventh day after radiation;Group 7 were treated with cWPROL (high dose)from the seventh day after radiation;Group 8 were treated with Western medicine from the seventh day after radiation.On every experimental finished point, the blood serum and full-length esophagus of rats were kept for examination.The content of hydroxyl radical (OH·)and NO, and the activity of CAT and GSH-PX in rats' esophagus tissues were determined.The content of NO in rats' blood serum was also analyzed.Results The content of hydroxyl radical (OH·)and NO and in Wistar rats' esophagus tissue increased significantly compared with that of the normal rat on the 14th day radiated by 43Gy 60Co-γ (P<0.05).The activity of CAT and GSH-PX increased significantly (P<0.01).However, the content of hydroxyl radical (OH·)and NO, and the activity of CAT and GSH-PX in the esophagus tissue of rat treated with cWPROL decreased significantly compared with that of the rat in radiated group 2 (P<0.05), the rat treated with cWPROL of high dose from the seventh day after radiation (P<0.01).The content of NO in blood serum was no significantly different among all of the groups (P>0.05).The content of NO and the activity of CAT and GSH-PX in the esophagus tissue were reduced in the rats treated with Western medicine (P<0.05)compared with that of the rats in radiation group 2, especially the marked decrease in the content of NO (P<0.01).Conclusion cWPROL can reduce the content of hydroxyl radical (OH·)and NO the activity of GSH-PX and CAT in esophagus tissue of rat radiated with 60Co-γ ray, which maybe is the mechanism for the treatment of inflammatory esophagus induced by radiation.
  • Radioprotective Effects of Shark Cartilage Mucopolysaccharide Preparation on Immune and Reproductive Organs in Male mice
    LUAN Jie, SHEN Xian-rong, JIANG Ding-wen, et al
    2008, 17(2): 145-147. https://doi.org/10.13491/j.cnki.issn.1004-714x.2008.02.052
    Download PDF ( ) HTML   Knowledge map   Save
    Objective To evaluate the protective effects of shark cartilage mucopolysaccharide preparation (SCMP)on immune and reproductive organs in male mice with irradiation damage induced by γ-rays.Methods 50 mice were randomly divided into normal control group, model control group, the 0.5g/kg·d SCMP group, the 1.0g/kg·d SCMP group, and the 2.0g/kg·d SCMP group.SCMP was administrated by intragastric infusion with the volume of 0.4ml per 20g.Normal control group and model control group were given the same volume of water.2 weeks later, all of the mice were irradiated by γ-ray of 60Co (0.83Gy/h)with the dose of 5 Gy.Peripheral blood WBC, the spleen index (SI), thymus index (TI), the BMNC, testicle index, germ cells were detected.Results Compared with the model control group, peripheral blood WBC became significantly higher in the treated groups with 1.0g/kg·d and 2.0g/kg·d SCMP after 3 days by irradiation (P<0.05).TI, BMNC and germ cells were significantly higher in the treated groups with 1.0g/kg·d and 2.0g/kg·d SCMP comparing with the control model group (P<0.05)and SI, and Testicle index was significantly compared with the model group (P<0.05)only in 2.0g/kg·d SCMP treated group.The germ abnormality rate became lower in SCMP treated groups (P<0.01)in 1.0 and 2.0g/kg·d SCMP group.Conclusion SCMP has radioprotective effects on immune and reproductive organs in male mice.
  • Antitumor Effects of Recombinant Adenovirus-Rb94 Gene Therapy and Radiotherapy on Growth of Human Hepatic Carcinoma cells
    ZHANG Yao-wen, WANG Qin, LI Jin, et al
    2008, 17(2): 147-149. https://doi.org/10.13491/j.cnki.issn.1004-714x.2008.02.068
    Download PDF ( ) HTML   Knowledge map   Save
    Objective To evaluate the effects of recombinant adenovirus-Rb94 and radiotherapy on the growth of human hepatic carcinoma cells.Methods Human hepatic carcinoma cells HepG2 were treated with Ad-Rb94, radiotherapy or Ad-Rb94 combined with radiotherapy respectively.The cell growth curve, cell cycle and cell apoptosis of HepG2 cells were examined.Rusults The growth of HepG2 cells transfected with Ad-Rb94, radiation and combination of Ad-Rb94 and radiation was significantly slower than that of Ad-lacZ and blank control (P<0.05), especially at 96h after transfection.The combination of Ad-Rb94 and radiation resulted in greater inhibition of cell growth compared with Ad-Rb94 infection and radiation at 96h after transfection (P<0.05).The number of cells of G2/M phases transfected with Ad-Rb94 and radiation increased, while the number cells of G0/G1 and S phases decreased;cells of apoptosis increased.The increase of cells of G2/M phases and apoptosis for the combination of Ad-Rb94 and radiation were the highest.Conclusion Ad-Rb94 gene therapy combined with radiotherapy show synergism for the inhibition of HepG2 cells growth.
  • Effects of Ion Implantation on Free Radicals Forming and Scavenging of Deinoeoecus Radiodurans
    LV Ping, YANG Liu-zhi
    2008, 17(2): 149-150. https://doi.org/10.13491/j.cnki.issn.1004-714x.2008.02.004
    Download PDF ( ) HTML   Knowledge map   Save
    Objective To study of the effects of N+ ion implantation on free radicals forming and scavenging were investigated in Dr (Deinococcus radiodurans)and the relationship between AFR (the amount of free radicals)and DNI (dose of N+ implantation)in Dr.Methods AFR in Dr was studied with ESR after N+ implantation and γ-radiation.Results It is proved that AFR in Dr ascended at first, then descended after reaching the peak, finally ascended again with increase of DNI.In high dose, AFR descended after Dr irradiated.while in low dose, AFR ascended.Conclusion scavenging effects of Dr against free radicals from high DNI were stronger than that from low DNI.
  • Protective Effect Study of E838 on Hematopoietic Function in Radiation-exposed Mice
    WANG Yue-ying, LI De-guan, WU Hong-ying, et al
    2008, 17(2): 151-152. https://doi.org/10.13491/j.cnki.issn.1004-714x.2008.02.078
    Download PDF ( ) HTML   Knowledge map   Save
    Objective To study the protective effects of E838 on the hematopoietic function in radiation exposed mice。Methods Colony-forming unit of spleen (CFU-S), the nucleated cells in bone marrow (BMNC)and spleen index were used to investigated the protective effects of E838 on hematopoietic function in mice irradiated with 7.5 Gy.Results On the 9 th day after irradiation, the CFU-S, BMNC numbers and spleen index of mice treated with E838 became obviously higher compared with these in the negative control group (P<0.001).The numbers of BMNC in the three E838 groups were higher than these in nilestrio group (P<0.001), compared with ethinyl estradiol group, the numbers of BMNC in middle and high dose E838 group increased (P<0.05).The spleen index in the three E838 groups were significantly greater than those both in negative control group and in nilestrio group (P<0.001).Conclusion The results showed that E838 could reduced the hematopoietic damage induced by irradiation.
  • The Effects of Three Types of Macrophages Culture Supernatant on CFU-GM in Irradiated Mice
    QUAN Hong-xun, FU Li, ZHAO Feng-chen, et al
    2008, 17(2): 153-154. https://doi.org/10.13491/j.cnki.issn.1004-714x.2008.02.053
    Download PDF ( ) HTML   Knowledge map   Save
    Objective To study the effects of peritional macrophyge (PM), alveolar macrophage (AM), and Kupffer cell (KC)on colony forming unite granulacyte/macrophage (CFU-GM)in irradiated mice.Methods Using techniques of hemopoietic progenitors in vitro, the authors studied the effects of three types of macrophages culture supernatant on CFU-GM.Results It is shown that three types of macrophages culture supernatant may stimulate proliferation and differentiation of CFU-GM in irradiated mice, and KC is the best one in comparison to others.Conclution three types of macrophages culture supernatant may protect CFU-GM irradiated mice with KC being the best method.
  • Analysis of the Correlation between dry residual and Total Radioactivity in Waters
    ZHANG Rui-ju, TU Yu
    2008, 17(2): 155-156. https://doi.org/10.13491/j.cnki.issn.1004-714x.2008.02.005
    Download PDF ( ) HTML   Knowledge map   Save
    Objective To analyzes the correlation between dry residual and total radioactivity in Waters.Methods We selected different waters in Suzhou to be studied.The gross alpha and gross beta activities in waters were measured with mid-thickness relative activity method associated with the normal source of 241Am and high purity KCl by using a low level alpha/beta counting system model BH1216.Results For the samples, correlation between dry residual and gross alpha activities was more statistically significant (P﹤0.01)than correlation between dry residual and gross beta activities (0.01

    <0.05).Their correlation values were 0.517 and 0.435, respectively.Conclusions The general rule, the lower the total dissolve solids (dry residual or conductivity values), the lower the radioactivity.We should standardize the traditional methods of water purification, aiming at the quantitative elimination of the presence in solution of the radionuclides.

  • Effects of Long-term,Low Dose Rate Fission Neutron Irradiation on the Reproductive System of Male Rats
    MA Li, LEI Cheng-xiang, SHEN Xian-rong, et al
    2008, 17(2): 157-159. https://doi.org/10.13491/j.cnki.issn.1004-714x.2008.02.023
    Download PDF ( ) HTML   Knowledge map   Save
    Objective To investigate the effects on germ system of male rats exposed to a low dose rate neutron irradiation.Methods Male rats were randomly divided into the control group and the irradiation group with low dose rate fission neutron (252Cf, 0.35mGy/h)irradiation.8 rats of each group were killed on 14d, 28d, 42d, 56d, 70d after irradiation and 35d after ceasing irradiation, and their abnormal sperms、testis/epididymis organ indexes andserum levels of testosterone were tested respectively.Results Compared with the control group, the abnomal sperm rate were evidently higher and the testis/epididymis organ indexes were evidently lower at the dose of 0.3Gy (P<0.05)。The serum levels of testosterone showed evident changes at 0.2Gy and 0.3Gy.No changes found at any other doses.Conclusion Low dose rate fission neutron affected the reproductive system at 0.2Gy and 0.3Gy, and due to the adaptability of rats, no cumulative effects was found.
  • The Correlative Study on the Pathological Alteration and Cytochrome c Oxidase Activity in Rat Lung Tissue Induced by Radon
    ZHANG Su-ping, XU Nai-yu, WU Zhao-zhao, et al
    2008, 17(2): 159-160,162. https://doi.org/10.13491/j.cnki.issn.1004-714x.2008.02.054
    Download PDF ( ) HTML   Knowledge map   Save
    Objective To observe the pathological change in rats exposed to radon, and to determine the cytochrome C oxidase activity in lung mitochondria.Methods Wistar rats were exposed to radon in a multifunction ecological radon chamber with the cumulative dose up to 100、200 and 400WLM respectively.The degree of lung injury was observed with HE staining and masson staining.The cytochrome C oxidase activity was determined by kit assay.Results In rats exposed to radon, a series of pathological alterations were observed including hyperaemia and edema in the alveolus interval, inflammatory cells such as lymphocytes were observed to penetrate mucous membrane and collagen fibre filled in interstitial of the alveolar.The enzyme activity was reduced in the mitochondria with the assay, and negative correlative to the thickness of alveolar septum, and positive correlative to the area of alveolar space.Conclusion The energy metabolism disorder may be induced by radon, Cytochrome C oxidase is probably one target of radon, and cell apoptosis in mitochondria maybe one of the pathways of radon biological effect.
  • The Clinical Significance of the Early Presence Hyperthyroidism from into Hyperthyroidism by Thyroid Antibody Due to the Radioiodine Treatment
    LIANG Hua-ju, MA Yu-qin, XU Yao-ling, et al
    2008, 17(2): 161-162. https://doi.org/10.13491/j.cnki.issn.1004-714x.2008.02.061
    Download PDF ( ) HTML   Knowledge map   Save
    Objective To observe the levels of TMAb, TGAb, TRAb in the serum of patients with hyperthyroidism before the treatment of radioiodine (131I)and investigate the clinical significance of the early hypothyroidism after 131I treatment.Methods According to the levels of thyroid auto-antibodies, 622 patients with hyperthyroidism were divided into four groups:group A 54 cases with negative TMAb, TGAb and TRAb, group B 176 cases with nagative TMAb and TGAb and positive TRAb, group C 78 caese with positive TMAb, TGAb and negative TRAb, Group D, 314 cases with positive TMAb TGAb and TRAb.The rate of early hypothyroidism were observed regularly after 131I therapy in each group.Results The rates of early hypothyroidism were 11.11% in group A, 13.64% in group B, 26.92% in group C and 23.57% in group D.Both the rates in group A and group B are lower than those in group C and group D (P<0.05).There is no difference in groups A.B.groups C and D (P>0.05).Conclusion The levels of TMAb, TGAb in are serum in an important influencing factor on the rate of the early hypothyroidism after 131I therapy for patients with hyperthyroidism, while the level of TRAb is of no importance.
  • The Prospective Study of the Effect of the Low-dosage Radiation on the Health of the Staff Members
    ZHANG Shi-cheng, TIAN Guang. LIU Lin-xiu, et al
    2008, 17(2): 163-165. https://doi.org/10.13491/j.cnki.issn.1004-714x.2008.02.024
    Download PDF ( ) HTML   Knowledge map   Save
    Objective To study the effect of the long term low-dosage radiation on the health of the staff members in order to provide a scientific basis for the rational protective measures to be taken.Methods Dynamic observations were made for 15 years of the conditions of the staff members exposed to radiation.The inherent changes and the affecting factors were analyzed.Group of people free from radiation were chosen to the control group.Results Sighs of nervous breakdown, damages of the eye crystal, hand skin and nails are much more frequently seen among medical radiation workers than these in the control group.There are differences between radiation workers and the control in the positive rates of the objective indices such as leukocytes, erythrocytes, blood platelets, immune functions, the minute nuclei and the chromosome fission.Conclusion Persistent low-dose radiation can cause damages to the health of radiation workers in many respects, Measures of radiation protection and persistant health monitoring should be taken.Thisis of great importance in implementing the state's relative laws and regulations to protect the health of the radiation workers.