WANG Ming-suo, DU Ze-ji, YANG Zhan-shan, et al
2000, 9(3): 137-138.
The exploration of the role of DNA-binding proteins (DBPs) from a radioresistant bacterium i.e.Deinococcus radiodurans(D.radiodurans).The DBPs from three wild type of D.radiodurans i.e.MR1, KR1 and Sark were determined by south-western bloting using the chromosomal DNA labeled with digoxin as a probe, and the N-terminal AA sequence of some DBPs was analysed.Results showed that there are 8 kinds of characterictic DBPs in the wild types of radioresistent bacterium whose molecular weights were 122, 93, 33, 29, 16, 15, 14and 12 kDa, among whom, the DBPs of 93 and 33kDa from wild type of KR1 were found to be novel proteins by the AA Sequence analysis.The expression levels of the two DBPs change with different culture media at different culture time.It was suggested that the DBPs, especially the DBPs of 93 and 33 kDa, were closely related to the radioresistance of D.radiodurans.